Abstract: The interacting of MinD and MinC and MinE proteins of Escherichia coli are required for proper placement of the division septum at midcell, The MinD protein plays a key role in the course of bacterial cell and plant chloroplast division. The over production of MinD protein can block the normal cell division and lead to a filamentation cell . In plant cells, There are clear similarities between bacterial and plastid division, but limited information exists regarding the mechanism of plastid division in higher plants . Here we constructed a expression plasmid encoding the full-length CrMinD, an
Chlamydomonas reinhardtii homologue of the bacterial MinD . In agreement with cell division studies in bacteria over expression of CrMinD in E. coli results in filamentation cell formation . The sesuggestion that CrMinD is an evolutionary conserved cell division protein .Meanwhile , the result lays a certain foundation for studying on the division mechanism of plant chloroplast.

Key words: Chloroplast